Q74: Which of the following gene silencing techniques involves the use... - Molecular Biology and Biotechnology - Bioinformatics- ICAR JRF/SRF 2024 | Agrigyan

• Small Non-Coding RNA Molecules in Gene Regulation:
• RNA interference (RNAi): The overarching pathway of sequence-specific post-transcriptional gene regulation mediated by short non-coding regulatory RNAs.
• MicroRNAs (miRNAs): Endogenously expressed ~21-23 nt non-coding RNAs that bind imperfectly to the 3' UTR of target mRNAs, causing translational repression or mRNA destabilization.
• Antisense RNA: Synthetic or natural regulatory RNA molecules complementary to mRNA transcripts that block translation or recruit RNase H.
• Small interfering RNAs (siRNAs): Exogenous or synthetic ~20-25 nt double-stranded RNA molecules that direct sequence-specific cleavage of target mRNAs via the RISC endonuclease Argonaute.
• Conclusion: All four techniques involve small RNA molecules: (A), (B), (C) and (D) (Option 3).

Correct option: (3) (A), (B), (C) and (D).