Q74: Thiobarbituric acid test used for the measuring of lipid oxidation... - Chemistry of Milk Lipids and Fat-Soluble Vitamins - Dairy Chemistry- ICAR JRF/SRF 2024 | Agrigyan
Thiobarbituric Acid (TBA) Assay for Lipid Oxidation:
Biochemical Basis:
Evaluates secondary lipid peroxidation in milk fat and meat tissues.
Measures malondialdehyde (MDA), a three-carbon dialdehyde cleaved from polyunsaturated fatty acid hydroperoxides.
Two molecules of 2-thiobarbituric acid condense with one molecule of malondialdehyde under acidic, boiling conditions to form a pink chromogen exhibiting maximal absorbance at $532\text{ nm}$.
Conclusion: Malanoaldehydes (Option 2).
Correct option: (2) Malanoaldehydes
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