RNA Interference: siRNA Mediated Gene Silencing, shRNA and Post-Transcriptional Gene Knockdown - Fish Biotechnology | Agrigyan

• RNA Interference (RNAi):
• First discovered in C. elegans by Andrew Fire and Craig Mello (1998, Nobel Prize 2006):
• RNA interference is an evolutionarily conserved post-transcriptional gene silencing mechanism where short double-stranded RNA molecules trigger sequence-specific mRNA degradation.
• Exogenous or endogenous double-stranded RNA is cleaved by the ribonuclease Dicer into $\approx 21-23\text{ bp}$ small interfering RNAs (siRNAs).
• siRNAs are loaded into the RISC (RNA-Induced Silencing Complex), unwound to a guide strand, and direct the endonuclease Argonaute (Ago2) to cleave complementary target mRNAs.
• nalysis:
• (RNA interference): Correct.

Correct option: (3) RNA interference