23
Ina typical T4 DNA ligase reaction, which of the following acts as a cofactor
EXPLANATION
Restriction Endonucleases, Modifying Enzymes
- Cofactor Specificity in DNA Ligases:
- T4 DNA Ligase (isolated from bacteriophage T4-infected E. coli):
- Requires Adenosine Triphosphate (ATP) and $Mg^{2+}$ as essential cofactors to form a phosphodiester bond between adjacent $5'$-phosphate and $3'$-hydroxyl ends.
- Reaction mechanism: Ligase-AMP intermediate formation via ATP hydrolysis, AMP transfer to $5'$-phosphate, and subsequent phosphodiester bond closure with release of free AMP.
- In contrast, native bacterial E. coli DNA ligase utilizes $NAD^+$ (nicotinamide adenine dinucleotide) rather than ATP.
- nalysis:
- (ATP): Correct.
Correct option: (1) ATP
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