Choose the correct answer from the options given below:
|
List - I
सूची - I
Generation of DNA sequencing
List-I (Generation of DNA sequencing
|
List - II
सूची - II
Method
List-II (Method
|
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|---|---|---|---|
| A | First generation First generation | I | HeliScope single molecule sequencer HeliScope single molecule sequencer |
| B | Second generation Second generation | II | Sanger method Sanger method |
| C | Third generation Third generation | III | Oxford Nanopore method Oxford Nanopore method |
| D | Fourth generation Fourth generation | IV | Illumina sequencing Illumina sequencing |
- First Generation $
ightarrow$ (II) Sanger method: Dideoxy chain termination sequencing using fluorescent ddNTP terminators and capillary electrophoresis.
- Second Generation (NGS) $
ightarrow$ (IV) Illumina sequencing: High-throughput sequencing-by-synthesis utilizing reversible dye terminators on clonal flow-cell clusters.
- Third Generation $
ightarrow$ (I) HeliScope single molecule sequencer: Pioneer single-molecule real-time sequencing platform (Helicos BioSciences) without preliminary clonal PCR amplification.
- Fourth Generation $
ightarrow$ (III) Oxford Nanopore method: Direct single-molecule sequencing measuring disruptions in ionic current as native nucleic acid strands traverse biological protein nanopores.
Correct option: (2) (A) = (Hl), (B) - (IV), (C)- (), (D) = (tll)