Q63: Match List-I with List-II:Choose the correct answer from the options... - Molecular Biology Techniques - Microbiology- ICAR JRF/SRF 2024 | Agrigyan

• First Generation $

ightarrow$ (II) Sanger method: Dideoxy chain termination sequencing using fluorescent ddNTP terminators and capillary electrophoresis.

• Second Generation (NGS) $

ightarrow$ (IV) Illumina sequencing: High-throughput sequencing-by-synthesis utilizing reversible dye terminators on clonal flow-cell clusters.

• Third Generation $

ightarrow$ (I) HeliScope single molecule sequencer: Pioneer single-molecule real-time sequencing platform (Helicos BioSciences) without preliminary clonal PCR amplification.

• Fourth Generation $

ightarrow$ (III) Oxford Nanopore method: Direct single-molecule sequencing measuring disruptions in ionic current as native nucleic acid strands traverse biological protein nanopores.

Correct option: (2) (A) = (Hl), (B) - (IV), (C)- (), (D) = (tll)