1
Given below are two statements
Statement (I) : Diethylpyrocarbonate (DEPC) is used to inactivate RNases in buffers and on glassware
Statement (II) : Diethylpyrocarbonate (DEPC) is a transition state analog that binds to active sites of RNases.
In light of the above statements, choose the most appropriate answer from the options given below.
EXPLANATION
Electrophoresis, Centrifugation, Chromatograp
- Statement (I) is True: Diethylpyrocarbonate (DEPC) is a strong chemical modifier routinely used to treat water, buffers, and glassware to eliminate ribonuclease (RNase) contamination before RNA isolation and manipulation.
- Statement (II) is False: DEPC is a chemical alkylating agent that carboethoxylates the active-site histidine residues (specifically $N^\epsilon$ or $N^\delta$ of the imidazole ring) of ribonucleases, causing irreversible covalent denaturation, NOT a non-covalent transition state analog.
- Conclusion: Statement (I) is true but Statement (II) is false.
Correct option: (3) Statement (I) is true but Statement (II) is false.
Explanation not matching this question?
क्या व्याख्या इस प्रश्न से मेल नहीं खा रही?
Report
Want to practice this paper in exam conditions?
क्या आप परीक्षा जैसे माहौल में अभ्यास करना चाहते हैं?
Test yourself in 5-question batch format with timer and hidden answers.
5-प्रश्नों के बैच फॉर्मैट, टाइमर और छिपे उत्तरों के साथ खुद को परखें।
Start Practice Mode →
प्रैक्टिस टेस्ट मोड शुरू करें →