1
Which of the following are features of Ti-plasmid?
(A) . They contain one (or more) T-DNA region
(B) . They contain a region enabling antibiotic resistance
(C) . They contain a vir region
(D) . They contain a region enabling conjugative transfer.
Choose the correct answer from the options given below:
EXPLANATION
Plant Transformation Systems
- Features of Natural Ti-Plasmid (Agrobacterium tumefaciens):
- T-DNA Region: Contains one or more transferred DNA regions transferred and stably integrated into the host plant nuclear genome.
- Virulence (vir) Region: Encodes Vir proteins (VirA, VirG, VirD1/D2, VirE2) required for processing, excising, and delivering T-DNA into the plant cell.
- Conjugative Transfer Region: Harbors tra/trb genes enabling plasmid transfer between agrobacterial cells via bacterial conjugation.
- Absence of Antibiotic Resistance (B): Wild-type Ti-plasmids do not harbor natural antibiotic resistance genes (they encode opine synthesis/catabolism and auxin/cytokinin biosynthesis). Antibiotic markers are added in engineered binary cloning vectors.
- Conclusion: (A), (C), and (D) only are correct.
Correct option: (2) (A), (C) and (D) only.
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