Q.1
Out of following, identify the most sensitive stain for proteins
- Sensitivity of Protein Stains in Polyacrylamide Gels (PAGE):
- Silver Stain: The most sensitive non-radioactive colorimetric stain, capable of detecting as little as $0.1\text{--}1\text{ ng}$ of protein per band. It relies on the binding of silver ions ($\text{Ag}^+$) to protein functional groups followed by chemical reduction to metallic silver ($\text{Ag}^0$).
- Coomassie Brilliant Blue (R-250 / G-250): Standard sensitivity, detecting approximately $10\text{--}50\text{ ng}$ of protein.
- Amido Black: Lower sensitivity, detecting approximately $50\text{--}100\text{ ng}$ of protein.
Correct option: (4) Silver stain
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