Q.1
Homopolymers can be added to DNA strands by using
EXPLANATION
Molecular Cloning Strategies: Restriction-Modification, Linkers/Adaptors & Directional Ligation
- Homopolymer Tailing in Recombinant DNA Technology:
- Terminal Deoxynucleotidyl Transferase (TdT): A template-independent DNA polymerase that adds successive deoxynucleotides to the $3'\text{-OH}$ end of DNA.
- When supplied with a single dNTP (e.g., dGTP or dCTP), TdT synthesizes a continuous homopolymeric tail (e.g., poly-dG on vector and poly-dC on insert cDNA), allowing complementary annealing and cohesive cloning without restriction enzyme cleavage.
- nalysis:
- (Terminal deoxynucleotidyltransferase): Correct enzyme.
Correct option: (2) Terminal deoxynucleotidyltransferase
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