Q.1
Which of the following steps is NOT an esstential process during cell cryopreservation?
EXPLANATION
Cryopreservation of Cells: Cryoprotectants (DMSO, Glycerol), Controlled Freezing & Thawing Kinetics
- Cell Cryopreservation Procedure:
- The standard protocol for cryopreserving cells comprises:
- Cell counting and assessment of viability ($\gt 90\%$) before freezing.
- Resuspension in cryopreservation medium containing a permeating cryoprotectant (e.g., $10\%$ DMSO) to prevent intracellular ice crystal formation.
- Gradual controlled-rate cooling (approximately $-1^\circ\text{C/min}$) from freezing down to ultra-low temperatures ($-80^\circ\text{C}$) before storage in liquid nitrogen ($-196^\circ\text{C}$).
- Rapid thawing at $37^\circ\text{C}$: A step performed during cell recovery/thawing from storage, not during the freezing/cryopreservation process itself.
Explanation not matching this question?
क्या व्याख्या इस प्रश्न से मेल नहीं खा रही?
Report
Want to practice this paper in exam conditions?
क्या आप परीक्षा जैसे माहौल में अभ्यास करना चाहते हैं?
Test yourself in 5-question batch format with timer and hidden answers.
5-प्रश्नों के बैच फॉर्मैट, टाइमर और छिपे उत्तरों के साथ खुद को परखें।
Start Practice Mode →
प्रैक्टिस टेस्ट मोड शुरू करें →