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Which of the following is a method for selective culture of tumor cells?
EXPLANATION
Bioreactors, Microcarriers, Scale-Up & Suspen
- Suspension Cloning (Soft Agar Assay):
- Normal adherent somatic cells require attachment to an extracellular matrix or solid substratum to grow (anchorage-dependent growth; detachment triggers anoikis).
- Malignant/tumor cells display anchorage-independent growth, enabling them to proliferate and form macroscopic colonies while suspended in semi-solid media like soft agar or methylcellulose.
- Suspension cloning is the benchmark method for the selective culture, isolation, and quantification of transformed tumor cells.
Correct option: (3) Suspension Cloning
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