Q.6
ICAR AIEEA PG Plant Biotechnology 14 July 2023 Shift-1 Paper (Q.67)
MCQ (Single Correct) +4 −1
English हिन्दी

The important features of Shine-Dalgarno sequence are:

(A) . Determine transcription initiation site
(B) . Distinct means of determining the translational start site in prokaryotes
(C) . Complementary to part of the 3' end of 16S rRNA
(D) . Termination recognition in prokaryotes

Choose the correct answer from the options given below:

शाइन-डालगार्नो अनुक्रम की महत्वपूर्ण विशेषताएं हैं:

(A) . अनुलेखन आरंभ स्थल निर्धारित करना
(B) . प्रोकैरियोट्स में अनुवादन आरंभ स्थल निर्धारित करने का विशिष्ट साधन
(C) . 16S rRNA के 3' सिरे के भाग का पूरक होना
(D) . प्रोकैरियोट्स में समापन की पहचान करना

नीचे दिए गए विकल्पों में से सही उत्तर का चयन कीजिए:

EXPLANATION Prokaryotic and Eukaryotic Gene Regulation, Operons and Epigenetics

Characteristics of the Shine-Dalgarno (SD) sequence:

  • False: Transcription initiation is determined by promoters (Pribnow box / -10 and -35 boxes), not by SD sequences.
  • True: In prokaryotic mRNAs, the SD sequence (consensus 5'-AGGAGG-3') is located 8 base pairs upstream of the AUG start codon and correctly positions the ribosome on the translation initiation codon.
  • True: The SD sequence base-pairs directly with the complementary anti-Shine-Dalgarno sequence (3'-UCCUCC-5') located at the 3'-end of the 16S rRNA of the 30S ribosomal subunit.
  • False: Translation termination is recognized by stop codons and release factors (RF1/RF2), not SD sequences.
Therefore, statements (B) and (C) only are correct.

शाइन-डालगार्नो अनुक्रम (SD sequence) के लक्षण:

  • असत्य: अनुलेखन आरंभ स्थल का निर्धारण प्रमोटर द्वारा होता है, SD अनुक्रम द्वारा नहीं।
  • सत्य: प्रोकैरियोटिक mRNA में SD अनुक्रम AUG आरंभिक कोडॉन से लगभग 8 बेस पहले स्थित होकर अनुवादन आरंभ स्थल को निर्धारित करता है।
  • सत्य: यह 30S राइबोसोम की 16S rRNA के 3' सिरे पर उपस्थित एंटी-शाइन-डालगार्नो अनुक्रम का पूरक होता है।
  • असत्य: समापन की पहचान स्टॉप कोडॉन द्वारा होती है।
अतः केवल कथन (B) और (C) सही हैं।
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