Q117: Which of the following molecular marker techniques is used for... - Cell Biology, Genetics, Physiology, Biochemistry & Plant Protection - ICAR AIEEA PG Plant Science 14 July 2023 Shift-1 Paper | Agrigyan

Molecular marker principles:

• Restriction Fragment Length Polymorphism (RFLP) is a hybridization-based marker that detects differences in homologous DNA fragment lengths generated by restriction enzyme digestion due to mutations that create or abolish restriction endonuclease recognition sites.
• Digested DNA is separated by agarose gel electrophoresis, transferred to membranes by Southern blotting, and visualized using radioactive or chemiluminescent probes.
• Thus, RFLP is the quintessential marker based on restriction fragment length differences.