Q80: The first amino acid incorporated in any nascent polypeptide chain... - Genetics, Plant Pathology, Seed Science & Agricultural Microbiology - ICAR AIEEA PG Plant Science 14 July 2023 Shift-1 Paper | Agrigyan

Translation initiation in eukaryotes:

• The universal initiation start codon is AUG on mRNA.
• In eukaryotes, the initiator tRNA ($tRNA_i^{Met}$) carries unformylated Methionine (Met), establishing methionine as the first amino acid at the N-terminus of all nascent eukaryotic polypeptides.
• In prokaryotes, the initiating residue is N-formylmethionine (fMet).