Microbial Energetics & Membrane Transport: PEP-PTS Systems, ABC Transporters & PMF - Dairy Microbiology | Agrigyan

1

Dairy Microbiology- ICAR JRF/SRF 2024 (Q.55)

MCQ (Single Correct) +4 −1

Main driving force in the dialysis process is

Pressure Electric potential Concentration Chemical potential

2

Dairy Microbiology- ICAR JRF/SRF 2024 (Q.96)

Statement Based +4 −1

Given below are two statements

Statement (I) : Carbohydrates are utilized by both a lactose PTS and lactose permease/8-galactosidase system in lactic acid bacteria

Statement (II) : Lactococcus lactis is one such bacteria that has both of the above system for lactose utilization

In light of the above statements, choose the most appropriate answer from the options given below.

Both Statement (I) and Statement (II) are true. Both Statement (I) and Statement (II) are false. Statement (I) is true but Statement (II) is false. Statement (I) is false but Statement ({I) is true.

3

Dairy Microbiology- ICAR JRF/SRF 2024 (Q.97)

MCQ (Single Correct) +4 −1

Which of the following nutrient transport systems require energy?
A) PEP:PTS system
B) Facilitated diffusion
C) Antiport
D) ABC transport system
Choose the correct answer from the options given below:

(A), (6) and (D) only. (A), (B) and (C) only, (B), (C), and (D) (A), (C) and (D) only.

General Agriculture

6 Topics

Agronomy, Tillage & Field Crop Production Agricultural Entomology, Nematology & Crop Protection Soil Science, Plant Nutrition & Reclamation Cell Biology, Plant Physiology & Biochemistry Agricultural Meteorology & Climate Science Crop Genetics, Plant Breeding & IPR

Scope and History of Microbiology

5 Topics

Bacterial Cell Ultrastructure: Peptidoglycan Architecture, Teichoic Acids & Periplasmic Transport Microbial Energetics & Membrane Transport: PEP-PTS Systems, ABC Transporters & PMF Active Microscopy in Dairying: Light, Fluorescence, Confocal, SEM, TEM & Specimen Preparation Microbial Taxonomy & Phylogeny: Polyphasic Taxonomy & 16S rRNA Molecular Classification Bacterial Growth Kinetics: Growth Phases, Specific Growth Rate, Generation Time & Chemostats

Microbial Genetics

5 Topics

Recombinant DNA Technology, PCR Primers & Metagenomics: DGGE & Amplicon Sequencing Molecular Genetics of Dairy Microorganisms: Central Dogma, Replication, Transcription & Translation Mutation & DNA Repair: Missense, Nonsense, Frameshift Mutations & Excision Repair Systems Horizontal Gene Transfer in Dairy Microbes: Conjugation, Transduction & Transformation Plasmids, Transposons & Phage Resistance: Dairy Plasmids (pSN2, pTRK2030) & Abortive Infection

Microbiological Quality of Milk

6 Topics

Raw Milk Microflora & Spoilage Dynamics: Succession from Bactericidal Stage to Acidification Milk-Borne Bacterial Pathogens & Zoonoses: Campylobacter jejuni, Listeria, E. coli O157:H7 & S. aureus Thermoduric & Thermophilic Microorganisms: Microbacterium, Sporeformers & 55°C Incubation Dairy Biofilms & Surface Adhesion: EPS Matrix, Quorum Sensing & CIP Sanitation Resistance Microbial Defects in Concentrated & Dried Milks: Mold Buttons, Bloat (Clostridium) & Bitterness Psychrotrophic Microflora & Cold-Chain Spoilage: Pseudomonas Heat-Stable Lipases & Proteases

Microbiology of Processed Foods

5 Topics

Food Biopreservation: Bacteriocins, Nisin, Pediocin, Mode of Action & GRAS Regulatory Status Radiation Microbiology: Non-Ionizing (UV, IR) vs Ionizing (Gamma, E-Beam) & Radiation Resistance Packaging Microbiology: Modified Atmosphere Packaging (MAP), Active Scavengers & Vacuum Spoilage Hurdle Technology & Intermediate Moisture Foods: Multi-Target Preservation & Homeostasis Milk Protective Systems & Bioactive Peptides: Lactoferrin, LP System & Antimicrobial Peptides

Starter Cultures and Probiotics

6 Topics

Cheese Microbiology & Ripening Flora: Starter vs NSLAB, Late Gas Defect & Flavor Development Probiotics Selection & Gastrointestinal Survival: Acid/Bile Tolerance (F1F0-ATPase) & FSSAI Standards Flavor Biogenesis in Fermented Milks: Diacetyl, Acetaldehyde (Threonine Pathway) & Acetoin Dairy Yeasts & Molds: Geotrichum candidum, Kluyveromyces & Ripening Fungi Lactic Acid Bacteria (LAB) Taxonomy & Hexose Fermentation: Homofermentative vs Heterofermentative Dairy Starter Culture Propagation: Media (Litmus Chalk Milk), Phage Control & Strains

Quality Assurance

5 Topics

Indicator Organisms of Dairy Sanitation: Total Coliforms, Fecal Coliforms, E. coli & Enterococci Microbiological Sampling Plans & Statistical Standards: Two-Class, Three-Class Plans & FSSAI Limits Rapid Detection & Biosensors in Dairy Microbiology: Optical, SPR, Piezoelectric & Impedance Sensors Commercial Sterility & Aseptic Quality Assurance: Sterility Testing, Incubation Protocols & Spores Biosafety in Dairy Laboratories: Biosafety Levels (BSL-1 to BSL-4) & M. tuberculosis Containment

Fermentation Technology

5 Topics

Bioreactor Design & Operating Modes: Batch, Fed-Batch, Chemostats & Continuous Fermentation Industrial Dairy Bioproducts & Enzymes: Microbial Rennet, Beta-Galactosidase & Biomass Production Fermentation Pathways & Bioenergetics: Alcoholic, Lactic & Mixed Acid Pathways, Pyruvate Fate Diagnostic Selective & Differential Media: Baird-Parker, XLD, EMB & Biochemical Identifications Solid State (SSF) vs Submerged Fermentation (SmF): Aeration, Energy Consumption & Downstream

Dairy Microbiology- ICAR JRF/SRF 2024

120 Questions Bilingual

RPSC Agriculture Exams

4 Exams

Assistant Agriculture Officer Agriculture Officer RPSC Agriculture Research Officer RPSC AARO

RSSB Agriculture Exams

1 Exam

RSSB Agriculture Supervisor

National & PG Entrances

4 Exams

ICAR AIEEA PG CUET UG Rajasthan Agriculture Pre-PG ICAR JRF/SRF

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