Q85: Limitations of microsatellites - Biochemical and Molecular Markers - Fish Biotechnology- ICAR JRF/SRF 2024 | Agrigyan

• Limitations of Microsatellite Markers:
• Null Alleles: Arise due to point mutations or deletions within the primer-annealing flanking regions, preventing PCR amplification. Heterozygotes are mistakenly scored as homozygotes, causing apparent heterozygote deficiency.
• Stutter Bands: In vitro slippage of Taq DNA polymerase during PCR amplification generates minor shadow or artifact bands (differing by multiples of the repeat motif length), complicating automated allele calling.
• nalysis:
• (Null alleles and Stutter bands): Correct.

Correct option: (4) Null alleles and Stutter bands