Q.1
Given below are two statements
Statement (I) : Diethylpyrocarbonate (DEPC) is used to inactivate RNases in buffers and on glassware
Statement (II) : Diethylpyrocarbonate (DEPC) is a transition state analog that binds to active sites of RNases.
In light of the above statements, choose the most appropriate answer from the options given below.
- Statement (I) is True: Diethylpyrocarbonate (DEPC) is a strong chemical modifier routinely used to treat water, buffers, and glassware to eliminate ribonuclease (RNase) contamination before RNA isolation and manipulation.
- Statement (II) is False: DEPC is a chemical alkylating agent that carboethoxylates the active-site histidine residues (specifically $N^\epsilon$ or $N^\delta$ of the imidazole ring) of ribonucleases, causing irreversible covalent denaturation, NOT a non-covalent transition state analog.
- Conclusion: Statement (I) is true but Statement (II) is false.
Correct option: (3) Statement (I) is true but Statement (II) is false.
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