Q116: Which sequencing technology uses fluorescently labeled nucleotides with reversible terminators? - Molecular Biology and Recombinant DNA Methods - Veterinary Biotechnology- ICAR JRF/SRF 2024 | Agrigyan

• Illumina Sequencing-by-Synthesis (SBS):
• Employs fluorescently labeled dNTPs containing reversible terminators:
• Each of the four dNTPs carries a distinct fluorescent dye attached via an enzymatically or chemically cleavable linker and a $3'\text{-O-azidomethyl}$ blocking group (reversible terminator).
• During each cycle, DNA polymerase incorporates only one complementary nucleotide. Fluorescence emission is imaged across the flowcell, after which the dye and terminator group are chemically cleaved, exposing a free $3'\text{-OH}$ for the next cycle.
• Other platforms: Ion Torrent detects $\text{H}^+$ ion release, PacBio uses zero-mode waveguides, and Nanopore measures ionic current changes through nanopores.

Correct option: (1) Illumina sequencing