Q48: What is the nature of stationary phase and mobile phase... - Food Chemistry, Nutrition, Food Additives & Analytical Techniques - ICAR AIEEA PG 2024 - Food Science & Technology | Agrigyan

In reversed-phase high-performance liquid chromatography (RP-HPLC):
The stationary phase is hydrophobic/non-polar (typically silica modified with bonded alkyl chains such as C18 octadecylsilane or C8).
The mobile phase is hydrophilic/polar (e.g., mixtures of water/buffer with organic modifiers like methanol or acetonitrile).
In normal-phase HPLC, the stationary phase is polar and mobile phase is non-polar.